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Fast parallel proteolysis : ウィキペディア英語版 | Fast parallel proteolysis
Fast parallel proteolysis (FASTpp) is a method to determine the thermostability of proteins that does not need previous purification and labelling. == History and background ==
Proteolysis is widely used in biochemistry and cell biology to probe protein structure. In "limited trypsin proteolysis", low amounts of protease digest both folded and unfolded protein but at largely different rates: unstructured proteins are cut more rapidly, while structured proteins are cut at a slower rate (sometimes by orders of magnitude). Recently, several other assays of protein stability based on proteolysis have been proposed, exploiting other proteases with high specificity for cleaving unfolded proteins. These include Pulse Proteolysis, Proteolytic Scanning Calorimetry and FASTpp.
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